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BCAP31 antibody [CC-1]

Anti-BCAP31 antibody [CC-1] used in IHC (Frozen sections) (IHC-Fr). GTX15044
Anti-BCAP31 antibody [CC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15044
Anti-BCAP31 antibody [CC-1] used in Western Blot (WB). GTX15044
Anti-BCAP31 antibody [CC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15044
Anti-BCAP31 antibody [CC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15044

Cat. No. GTX15044

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 CC-1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, IP, ELISA

Reactivity

 Human, Mouse, Bovine, Hamster, Primate
Package
100 μl ($399)
See all BCAP31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,500 - 1:6,000
ICC/IF 1:200-1:1000
IHC-Fr 1:1000 - 1:10000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Amino acid residues 230-246 of BAP31 protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B cell receptor associated protein 31 , 6C6-AG , BAP31 , CDM , DDCH , DXS1357E

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-BCAP31 antibody [CC-1] used in IHC (Frozen sections) (IHC-Fr). GTX15044

GTX15044 IHC-Fr Image

IHC-Fr analysis of baboon adrenal gland tissue using GTX15044 BCAP31 antibody [CC-1].

Anti-BCAP31 antibody [CC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15044

GTX15044 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15044 BCAP31 antibody [CC-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:200 incubated overnight at 4ºC

Anti-BCAP31 antibody [CC-1] used in Western Blot (WB). GTX15044

GTX15044 WB Image

WB analysis of samples using GTX15044 BCAP31 antibody [CC-1].

Anti-BCAP31 antibody [CC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15044

GTX15044 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX15044 BCAP31 antibody [CC-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:200 incubated overnight at 4ºC

Anti-BCAP31 antibody [CC-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15044

GTX15044 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15044 BCAP31 antibody [CC-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:200 incubated overnight at 4ºC

REFERENCE

Clone Reference

P Walch et al. Cell Host Microbe 2021; 29 (8):1316-1332.e12 Global mapping of Salmonella enterica-host protein-protein interactions during infection
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399