Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BMAL1 antibody [N1N3] detects BMAL1 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BMAL1 protein stained by BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot analysis of BMAL1 expression in transfected 293T cell line by BMAL1 polyclonal antibody. A: Non-transfected lysate. B: BMAL1 transfected lysate. 7.5% SDS PAGE GTX105060 diluted at 1:500

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105060 diluted at 1:1000