HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100874) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using BMP4(GTX100874) antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4(GTX100874) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min