Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. Control with 2.5 μg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 μg of BRE antibody (GTX105364) 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody (GTX105364) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

BRCC45 antibody detects BRE protein by Western blot analysis.
A. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (GTX105364) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE(GTX105364) antibody at 1:200 dilution.

BRCC45 antibody detects BRE protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (GTX105364) dilution: 1:1000

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.