Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody (GTX130586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

293T whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody (GTX130586) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of BRD4 protein from 293T whole cell extract using 5 μg of BRD4 antibody (GTX130586).
Western blot analysis was performed using BRD4 antibody (GTX130586).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody (GTX130586) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

BRD4 antibody detects BRD4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

BRD4 antibody detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BRD4 antibody detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BRD4 antibody detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min