Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using BUB1(GTX107497) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BUB1 antibody (GTX107497) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)

A: A431 (GTX27909)

7.5% SDS PAGE

GTX107497 diluted at 1:3000

BUB1 antibody detects BUB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
5 % SDS-PAGE
BUB1 antibody (GTX107497) dilution: 1:1000