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Bad antibody

KO/KD Orthogonal Overexpression
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in IHC (Paraffin sections) (IHC-P). GTX130108
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in Western Blot (WB). GTX130108
Anti-Bad antibody used in Western Blot (WB). GTX130108

Cat. No. GTX130108

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Bad products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

293T , A431 , HeLa , BAD-transfected 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bad. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated agonist of cell death , BBC2 , BCL2L8

Cellular Localization

Mitochondrion outer membrane , Cytoplasm

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

KO/KD

Wild-type (WT) and Bad knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bad antibody used in IHC (Paraffin sections) (IHC-P). GTX130108

GTX130108 IHC-P Image

Bad antibody detects Bad protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bad stained by Bad antibody (GTX130108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bad antibody used in Western Blot (WB). GTX130108

GTX130108 WB Image

Bad antibody detects Bad protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bad antibody (GTX130108) diluted at a dilution of 1:1000.

REFERENCE

Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1) : 121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281
Wei GJ et al. AAPS J 2019; 21 (5) : 86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3) : 15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
Application : WB
Reactivity : Human
Lee CC et al. PLoS One 2017; 12 (3) : e0173890 the microrna-302b-inhibited *-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor ia.
Application : WB
Reactivity : Human
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REVIEW

Bad antibody Cat. No. GTX130108

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Bad antibody used in Western Blot (WB). GTX130108
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : untreated-30 μM cisplatin treatment for 24hr.
Submit a Review
Application Reference
Chua HH et al. Cell Mol Gastroenterol Hepatol 2023; 15 (1):121-152 PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
Application : WB
Reactivity : Human
Eid W et al. J Cell Biochem 2017; (Epub) neferine enhances the antitumor effect of *-c in hela cells through the activation of p38-mapk pathway.
Application : WB
Reactivity : Human
Lee CC et al. PLoS One 2017; 12 (3):e0173890 the microrna-302b-inhibited *-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor ia.
Application : WB
Reactivity : Human
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169