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Bad antibody

Orthogonal
Anti-Bad antibody used in IHC (Paraffin sections) (IHC-P). GTX50417
Anti-Bad antibody used in Western Blot (WB). GTX50417

Cat. No. GTX50417

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Bad products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide sequence around aa.153~157 (R-M-S-D-E) derived from mouse Bad.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167368

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated agonist of cell death , BBC2 , BCL2L8

Cellular Localization

Mitochondrion outer membrane,Cytoplasm

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bad antibody used in IHC (Paraffin sections) (IHC-P). GTX50417

GTX50417 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50417 Bad antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Bad antibody used in Western Blot (WB). GTX50417

GTX50417 WB Image

Orthogonal

WB analysis of extracts from 293 cells using GTX50417 Bad antibody with or without blocking peptide.

REFERENCE

Application Reference

Pu YF et al. Int J Clin Exp Pathol 2014; 7 (4) : 1563-1571 Generation of homologous cell pairs using the oral lymphatic system
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399