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Bad (phospho Ser136) antibody

Orthogonal
Anti-Bad (phospho Ser136) antibody used in IHC (Paraffin sections) (IHC-P). GTX50136
Anti-Bad (phospho Ser136) antibody used in Western Blot (WB). GTX50136
Anti-Bad (phospho Ser136) antibody used in Western Blot (WB). GTX50136

Cat. No. GTX50136

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Bad products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide sequence around phosphorylation site of serine136 (S-R-S(p)-A-P) derived from mouse Bad.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11167624

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2-associated agonist of cell death , AI325008 , Bbc2

Cellular Localization

Mitochondrion outer membrane,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Bad (phospho Ser136) antibody used in IHC (Paraffin sections) (IHC-P). GTX50136

GTX50136 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50136 Bad (phospho Ser136) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Bad (phospho Ser136) antibody used in Western Blot (WB). GTX50136

GTX50136 WB Image

Orthogonal

WB analysis of extracts from HT-29 cells untreated or treated with UV using GTX50136 Bad (phospho Ser136) antibody.

Anti-Bad (phospho Ser136) antibody used in Western Blot (WB). GTX50136

GTX50136 WB Image

The data was published in the journal Biomed Res Int in 2015. PMID: 26557697

REFERENCE

Application Reference

Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281
Vallejo-Flores G et al. Biomed Res Int 2015; 2015 : 761501 Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation.
Gabriela Vallejo-Flores et al. BioMed Research International 2015; Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation
Application : WB, IP
Reactivity : Human
Huang CY et al. Mol Neurobiol 2014; Novel Link of Anti-apoptotic ATF3 with Pro-apoptotic CTMP in the Ischemic Brain.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399