APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
human BCL2-transfected 293T cells
Predict Reactivity
Mouse, Rat, Bovine, Cat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888079
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 apoptosis regulator , Bcl-2 , PPP1R50
Cellular Localization
Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX628904 IHC-P Image
Bcl-2 antibody [GT576] detects Bcl-2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human tonsil.Bcl-2 stained by Bcl-2 antibody [GT576] (GTX628904) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX628904 WB Image
Bcl-2 antibody [GT576] detects Bcl-2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human Bcl-2-transfected 293T cells 12 % SDS-PAGE Bcl-2 antibody [GT576] (GTX628904) dilution: 1:5000
|
REFERENCE
There are currently no references for Bcl-2 antibody [GT576] (GTX628904). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Bcl-2 antibody [GT576] (GTX628904). Be the first to share your experience with this product.