Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Bcl-2 antibody detects Bcl-2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
Bcl-2 stained by Bcl-2 antibody (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PG-4 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Bcl-2 antibody detects Bcl-2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
Bcl-2 stained by Bcl-2 antibody (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Apoptosis in 2020. PMID: 32100210

The data was published in the journal Apoptosis in 2020. PMID: 32100210

The data was published in the journal Apoptosis in 2020. PMID: 32100210

The data was published in the journal Apoptosis in 2020. PMID: 32100210

The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23091557

The data was published in the journal PLoS One in 2016. PMID: 26859149

The data was published in the journal PLoS One in 2015. PMID: 25849560

The data was published in the journal Stem Cell Res Ther in 2017.PMID: 28482861

The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126

The data was published in the journal Front Pharmacol in 2017.PMID: 29326587

The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770

The data was published in the journal Biomolecules in 2020.PMID: 32635653

The data was published in the journal J Cell Mol Med in 2018. PMID: 29682889

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31210840