Wild-type (WT) and Beclin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin 1 antibody (GTX133555) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Beclin 1 antibody detects Beclin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Beclin 1 stained by Beclin 1 antibody (GTX133555) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min