WB analysis of various samples using GTX48808 Borrelia burgdorferi Erpd/Arp37 antibody.
Lane 1 : Protein ladder
Lane 2 : MBP-Erpd/Arp37 fusion protein
Lane 3 : MBP
Loading : 0.1 μg
Dilution : 1:1000

Western Blot showing detection of 0.1 μg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpD/Arp37 fusion proteins (arrow: expected MW of major band: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).