Negative controls are used to determine the specificity of an antibody. No staining should be observed when the primary antibody is omitted. Use GTX03597 Broad Spectrum Negative Control in place of the primary antibody with a section to evaluate nonspecific staining and allow better interpretation of staining results. This product is specifically designed to work with rabbit, mouse and goat antibodies. The Broad Spectrum Negative Control has been titered to work with Polymer-based Secondary Systems in manual and automated IHC platforms.