Sandwich ELISA detection of recombinant Human C Reactive Protein, His tag (GTX138453-pro) using antibodies as below.

Capture: C Reactive Protein antibody [GT54] (GTX641406) (5 μg/mL)
Detection: C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL)

Sandwich ELISA detection of recombinant Human C Reactive Protein, His tag (GTX138453-pro) using antibodies as below.

Capture: C Reactive Protein antibody [GT55] (GTX641407) (5 μg/mL)
Detection: C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL)

C Reactive Protein antibody [N1C3] detects secreted C Reactive Protein protein by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
C Reactive Protein stained by C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Human C Reactive Protein, His tag (GTX138453-pro) (144.14-2.25 nM). Coated protein was probed with C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.