Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with C16orf62 antibody (GTX107271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample(30 μg whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
7.5% SDS PAGE
GTX107271 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using C16orf62(GTX107271) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min