Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with C1qA antibody [HL1695] (GTX637294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1qA antibody [HL1695] (GTX637294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.