Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316).
Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C3 antibody [C3], C-term (GTX101316) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GTX101316) diluted at 1:1000.

C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200.
Blue: Hoechst 33342 staining.

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.