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C3a Receptor antibody [HL2831]

Recombinant antibody Orthogonal
Anti-C3a Receptor antibody [HL2831] used in Western Blot (WB). GTX640102
Anti-C3a Receptor antibody [HL2831] used in IHC (Paraffin sections) (IHC-P). GTX640102
Anti-C3a Receptor antibody [HL2831] used in IHC (Paraffin sections) (IHC-P). GTX640102
Anti-C3a Receptor antibody [HL2831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640102

Cat. No. GTX640102

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2831

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all C3a Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This antibody was raised against human C3a Receptor Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human C3a Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement C3a receptor 1 , AZ3B , C3AR , HNFAG09

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

C3a is an anaphylatoxin released during activation of the complement system. The protein encoded by this gene is an orphan G protein-coupled receptor for C3a. Binding of C3a by the encoded receptor activates chemotaxis, granule enzyme release, superoxide anion production, and bacterial opsonization. [provided by RefSeq, May 2016]

Database

Research Area

DATA IMAGES

Anti-C3a Receptor antibody [HL2831] used in Western Blot (WB). GTX640102

GTX640102 WB Image

Orthogonal

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C3a Receptor antibody [HL2831] used in IHC (Paraffin sections) (IHC-P). GTX640102

GTX640102 IHC-P Image

C3a Receptor antibody [HL2831] detects C3a Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C3a Receptor antibody [HL2831] used in IHC (Paraffin sections) (IHC-P). GTX640102

GTX640102 IHC-P Image

C3a Receptor antibody [HL2831] detects C3a Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded HL-60 xenograft.
C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C3a Receptor antibody [HL2831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640102

GTX640102 ICC/IF Image

C3a Receptor antibody [HL2831] detects C3a Receptor protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations