Rat plasma (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

C9 antibody [N2C2-2], Internal detects C9 protein at by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9 stained by C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:500.
Blue: Hoechst 33342 staining.

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal (GTX110161) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX110161 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.