FACS analysis of C6 cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue).
Dilution : 1:100 for 60 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of mouse skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

ICC/IF analysis of HeLa cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

WB analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates using GTX22864 CACNA2D1 antibody [20A].
Dilution : 1:1000

FACS analysis of Neuro-2a cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue).
Dilution : 1:100 for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of U251 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

IHC-P analysis of human skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

FACS analysis of SH-SY5Y cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue).
Dilution : 1:100 for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of PC-12 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC