ChIP analysis of sheared chromatin from 1.25x10⁶ SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. This figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
Antibody amount : 4μl

ChIP analysis of SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. The IP'd DNA from 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

ELISA analysis of peptides used for immunization using GTX60333 CBFb antibody - ChIP grade.