Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2416] (GTX638638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CCN3 antibody [HL2416] detects secreted CCN3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
CCN3 stained by CCN3 antibody [HL2416] (GTX638638) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min