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CCR1 antibody [HL3034]

Recombinant antibody Orthogonal Overexpression
Anti-CCR1 antibody [HL3034] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640472
Anti-CCR1 antibody [HL3034] used in Western Blot (WB). GTX640472
Anti-CCR1 antibody [HL3034] used in Western Blot (WB). GTX640472

Cat. No. GTX640472

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3034

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CCR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody was raised against human CCR1 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CCR1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine receptor 1 , CD191 , CKR-1 , CKR1 , CMKBR1 , HM145 , MIP1aR , SCYAR1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CCR1 antibody [HL3034] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640472

GTX640472 ICC/IF Image

Overexpression

CCR1 antibody [HL3034] detects CCR1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: CCR1 stained by CCR1 antibody [HL3034] (GTX640472) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CCR1 antibody [HL3034] used in Western Blot (WB). GTX640472

GTX640472 WB Image

Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL3034] (GTX640472) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCR1 antibody [HL3034] used in Western Blot (WB). GTX640472

GTX640472 WB Image

Orthogonal

A549 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL3034] (GTX640472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations