Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL3034] (GTX640472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

CCR1 antibody [HL3034] detects CCR1 protein by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CCR1 stained by CCR1 antibody [HL3034] (GTX640472) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL3034] (GTX640472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

CCR1 antibody [HL3034] detects CCR1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CCR1 stained by CCR1 antibody [HL3034] (GTX640472) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CCR1 antibody [HL3034] detects CCR1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: CCR1 stained by CCR1 antibody [HL3034] (GTX640472) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

A549 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL3034] (GTX640472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL3034] (GTX640472) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.