APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human CCR10 Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain human of CCR10. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine receptor 10 , GPR2
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CCR10 is the receptor for CCL27 (SCYA27; MIM 604833); CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (Homey et al., 2002 [PubMed 11821900]).[supplied by OMIM, Mar 2008]
Database
Research Area
DATA IMAGES
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GTX640123 Functional Assay Image
CCR10 antibody [HL2852] (GTX640123) specifically detects CCR10 protein on a VirDTM-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:1000 dilution.Yellow: CCR10White: Positive control
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GTX640123 WB Image
NCI-H929 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR10 antibody [HL2852] (GTX640123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX640123 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR10 antibody [HL2852] (GTX640123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX640123 ICC/IF Image
CCR10 antibody [HL2852] detects CCR10 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: CCR10 stained by CCR10 antibody [HL2852] (GTX640123) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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REVIEW
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