Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCR5 antibody [HL3650] (GTX641641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR5 antibody [HL3650] (GTX641641) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.