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CD11b antibody [OX-42]

Anti-CD11b antibody [OX-42] used in Flow cytometry (FACS). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060
Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

Cat. No. GTX76060

Reference 11

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OX-42

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Rat
Package
500 μg ($409)
See all CD11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1/50-1/100
FACS 1/50-1/100
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Resident rat peritoneal macrophages.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_379189

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Integrin Subunit Alpha M , Cd11B , Itgam

Research Area

DATA IMAGES

Anti-CD11b antibody [OX-42] used in Flow cytometry (FACS). GTX76060

GTX76060 FACS Image

FACS analysis of rat peritoneal macrophages using GTX76060 CD11b antibody [OX-42].

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD11b antibody (GTX76060), red in A and Mouse anti Rat CD8 (GTX41831), green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Medium power

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD11b antibody (GTX76060), red in A and Mouse anti Rat CD8 (GTX41831), green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX76060 CD11b antibody [OX-42].

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD11b antibody (GTX76060), red in A and Mouse anti Rat CD8 (GTX41831), green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Low power

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX76060 CD11b antibody [OX-42].

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX76060 CD11b antibody [OX-42].

Anti-CD11b antibody [OX-42] used in IHC (Frozen sections) (IHC-Fr). GTX76060

GTX76060 IHC-Fr Image

The data was published in the journal PLoS One in 2016. PMID: 26745377

REFERENCE

Application Reference

Li S et al. Mol Med 2022; 28 (1) : 133 Bone marrow mesenchymal stem cell-derived exosomes shuttling miR-150-5p alleviates mechanical allodynia in rats by targeting NOTCH2 in microglia.
Application : WB, IHC-P
Reactivity : Rat
Lee H.P. et al. ournal of Functional Foods 2021; 86 : 104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Application : WB
Reactivity : Human
Truzzi F et al. Int J Mol Sci 2020; 22 (1) Pro-Inflammatory Effect of Gliadins and Glutenins Extracted from Different Wheat Cultivars on an In Vitro 3D Intestinal Epithelium Model.
Application : IHC-P
Reactivity : Human
Bai N et al. J Neuroinflammation 2020; 17 (1) : 45 G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense.

Clone Reference

Aminzadeh A et al. Cell J 2020; 22 (Suppl 1):38-48 Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
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REVIEW

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Application Reference
Li S et al. Mol Med 2022; 28 (1):133 Bone marrow mesenchymal stem cell-derived exosomes shuttling miR-150-5p alleviates mechanical allodynia in rats by targeting NOTCH2 in microglia.
Application : WB, IHC-P
Reactivity : Rat
Lee H.P. et al. ournal of Functional Foods 2021; 86:104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Application : WB
Reactivity : Human
Truzzi F et al. Int J Mol Sci 2020; 22 (1) Pro-Inflammatory Effect of Gliadins and Glutenins Extracted from Different Wheat Cultivars on an In Vitro 3D Intestinal Epithelium Model.
Application : IHC-P
Reactivity : Human
Bai N et al. J Neuroinflammation 2020; 17 (1):45 G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense.
Lin CH et al. Cell Mol Neurobiol 2019; 39 (5):591-604 Microglia-Derived Adiposomes are Potential Targets for the Treatment of Ischemic Stroke.
Application : IHC-P
Reactivity : Rat
Bonfante R et al. Neuroscience 2018; 391:120-130 The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints.
Application : IHC-P
Reactivity : Rat
Lin CH et al. PLoS One 2016; 11 (1):e0146692 Molecular Mechanisms Responsible for Neuron-Derived Conditioned Medium (NCM)-Mediated Protection of Ischemic Brain.
Nakajima H et al. J Neurotrauma 2012; 29 (8):1614-25 Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury.
Application : IHC-Fr
Reactivity : Rat
Clone Reference
Aminzadeh A et al. Cell J 2020; 22 (Suppl 1):38-48 Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
Takahashi K et al. Eur J Pain 2011; 15 (5):467.e1-14 IL-1beta in the trigeminal subnucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury.
Zhao Z et al. Glycoconj J 2009; 26 (2):173-88 Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409