CD133 antibody [N1C1] detects CD133 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CD133 stained by CD133 antibody [N1C1] (GTX102109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD133 antibody [N1C1] detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [N1C1] (GTX102109) dilution: 1:5000

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.