Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD28 antibody [HL1589] (GTX637068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD28 antibody [HL1589] detects CD28 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog spleen.
CD28 stained by CD28 antibody [HL1589] (GTX637068) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD28 antibody [HL1589] detects CD28 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
CD28 stained by CD28 antibody [HL1589] (GTX637068) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD28 antibody [HL1589] detects CD28 protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD28 stained by CD28 antibody [HL1589] (GTX637068) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD28 antibody [HL1589] (GTX637068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.