Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD2AP antibody (GTX106235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP(GTX106235) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

CD2AP antibody detects CD2AP protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD2AP stained by CD2AP antibody (GTX106235) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD2AP antibody detects CD2AP protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD2AP antibody (GTX106235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using CD2-associated protein(GTX106235) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX106235 diluted at 1:1000