Jurkat whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD3 delta antibody (GTX105811) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.