Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000.

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using CD36(GTX100642) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CD36(GTX100642) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CD36 antibody [C1C3] detects CD36 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
CD36 stained by CD36 antibody [C1C3] (GTX100642) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Nutrients in 2020. PMID: 32164196

The data was published in the 2022 in Front Oncol. PMID: 35530322