Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody (GTX112891) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brown adipose
7.5% SDS PAGE
GTX112891 diluted at 1:1000

CD36 antibody detects CD36 protein at cytosol and weak membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
CD36 antibody (GTX112891) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CD36 antibody detects CD36 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
CD36 stained by CD36 antibody (GTX112891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 antibody (GTX112891) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody (GTX112891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.