ICC/IF analysis of Raji cells using GTX15669 CD58 antibody [TS2/9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

FACS analysis of BAF-3 cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

WB analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) using GTX15669 CD58 antibody [TS2/9].
Dilution : 1:100

FACS analysis of PBMC cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4ºC