CD66e antibody [1106]

Cat. No. GTX15677

Host	Mouse
Clonality	Monoclonal
Clone Name	1106
Isotype	IgG1
Application	WB, ICC/IF, FACS, IP, ELISA, RIA
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX15677 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1μg/ml
ICC/IF	Assay dependent
FACS	5-20μg/ml
IP	Assay dependent
ELISA	Assay dependent
RIA	Assay dependent

Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM Potassium Phosphate, 135mM NaCl, 2.5mM KCl, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified CEA from pooled tumors

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carcinoembryonic antigen related cell adhesion molecule 5 , CD66e , CEA

Cellular Localization

Cell membrane , Apical cell membrane , Cell surface

Background

This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Database

Gene ID: 1048 CEACAM5
UniProt: P06731 CEACAM5

Research Area

DATA IMAGES

GTX15677 WB Image

WB analysis of 20 ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well using GTX15677 CEA antibody [1106].
Dilution : 1 μg/ml

GTX15677 ICC/IF Image

ICC/IF analysis of BxPC-3 cells using GTX15677 CEA antibody [1106].
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 10 μg/ml in blocking buffer for 1 hour at room temperature

GTX15677 FACS Image

FACS analysis of BxPC-3 cells using GTX15677 CEA antibody [1106].
Pink histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml in PBS + 5% FCS

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty