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CD86 antibody

Anti-CD86 antibody used in Western Blot (WB). GTX32507
Anti-CD86 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32507

Cat. No. GTX32507

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody used in Western Blot (WB). GTX32507

GTX32507 WB Image

WB analysis of Daudi whole cell lysate using GTX32507 CD86 antibody.
Dilution : 1:2000
Loading : 25μg

Anti-CD86 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32507

GTX32507 ICC/IF Image

ICC/IF analysis of Raji cells using GTX32507 CD86 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

REFERENCE

Application Reference

Lombardo KA et al. J Pathol 2022; 256 (2) : 223-234 BCG invokes superior STING-mediated innate immune response over radiotherapy in a carcinogen murine model of urothelial cancer.
Hongmei Zhao et al. Bioengineered 2022; 13 (4) : 9928-9944 Sp1 transcription factor represses transcription of phosphatase and tensin homolog to aggravate lung injury in mice with type 2 diabetes mellitus-pulmonary tuberculosis.
Carbone F et al. Vascul Pharmacol 2016; 85 : 57-65 Vitamin D receptor is expressed within human carotid plaques and correlates with pro-inflammatory * macrophages.
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399