Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD90 antibody [HL1766] (GTX637416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

CD90 antibody [HL1766] detects CD90 protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD90 stained by CD90 antibody [HL1766] (GTX637416) diluted at 1:250.
Red: Tau, an Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CD90 antibody [HL1766] detects CD90 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: CD90 stained by CD90 antibody [HL1766] (GTX637416) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD90 antibody [HL1766] (GTX637416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.