Boiled and unboiled wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD97 antibody [HL1924] detects CD97 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in MeOH.
Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1924] (GTX637673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD97 antibody [HL1924] detects CD97 protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis.
Sample: U937 cells were fixed in ice-cold MeOH for 5 min.
Green: CD97 stained by CD97 antibody [HL1924] (GTX637673) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).