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CD97 antibody [HL1925]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in Immunoprecipitation (IP). GTX637674
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674
Anti-CD97 antibody [HL1925] used in IHC (Paraffin sections) (IHC-P). GTX637674

Cat. No. GTX637674

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1925

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD97 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Product Note

This antibody was raised against human CD97 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD97. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adhesion G protein-coupled receptor E5 , CD97 , TM7LN1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

KO/KD

Wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

KO/KD Comparison Orthogonal

Wild-type (WT) and CD97 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

Comparison Orthogonal

U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and competitor's antibody (Clone EPR4427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

Untreated (–) and treated (+) U937 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD97 antibody [HL1925] used in Immunoprecipitation (IP). GTX637674

GTX637674 IP Image

Immunoprecipitation of CD97 protein from U937 using 5 μg of CD97 antibody [HL1925] (GTX637674).
Western blot analysis was performed using CD97 antibody [HL1925] (GTX637674).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

Orthogonal

Boiled and unboiled U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-CD97 antibody [HL1925] used in Western Blot (WB). GTX637674

GTX637674 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD97 antibody [HL1925] used in IHC (Paraffin sections) (IHC-P). GTX637674

GTX637674 IHC-P Image

CD97 antibody [HL1925] detects CD97 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD97 stained by CD97 antibody [HL1925] (GTX637674) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations