Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC20 antibody (GTX111137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX111137 diluted at 1:1000

The data was published in the journal Nat Commun in 2016. PMID: 26743940