ICC/IF analysis of HeLa cells in G1 or early S phase using GTX00896 CDC6 antibody or Control non-immune antibody.
Green : control or primary antibody
Red : PI (propidium iodide)

ICC/IF analysis of MEF cells (E14.5) using GTX00896 CDC6 antibody. Note that CDC6 abundantly localizes in nuclei of the cells at G1 and early S phase.
Fixation : 4% PFA, RT, 10 min
Permeabilization : ice-cold methanol, on ice, 10 min
Dilution : 1:200

WB analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody.
Dilution : 1:1000
Loading : 10 μg

IP analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody.
Lane 1 : Control IP with non-immune IgG
Lane 2 : Immunoprecipitates with anti-CDC6 antibody
Lane 3 : Input