Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [HL1407] (GTX636873) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [HL1407] (GTX636873) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [HL1407] (GTX636873) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CDK4 antibody [HL1407] detects CDK4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
CDK4 stained by CDK4 antibody [HL1407] (GTX636873) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min