Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDK5(GTX108328) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108328 diluted at 1:1000

CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CDK5 antibody [C2C3], C-term (GTX108328) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody [C2C3], C-term (GTX108328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody (GTX108328) diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5(GTX108328) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min