Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N1C3] (GTX103992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N1C3] (GTX103992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CDK6 antibody [N1C3] detects CDK6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK6 stained by CDK6 antibody [N1C3] (GTX103992) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

CDK6 antibody [N1C3] detects CDK6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
CDK6 stained by CDK6 antibody [N1C3] (GTX103992) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CDK6 antibody [N1C3] detects CDK6 protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
CDK6 antibody [N1C3] (GTX103992) diluted at 1:500.

CDK6 antibody [N1C3] detects CDK6 protein at cytoplasm and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
CDK6 antibody [N1C3] (GTX103992) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N1C3] (GTX103992) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N1C3] (GTX103992) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal J Hematol Oncol in 2017.PMID: 28114995

The data was published in the journal J Hematol Oncol in 2017.PMID: 28114995