Wild-type (WT) and p14ARF knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody (GTX129903) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p14ARF antibody detects p14ARF protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p14ARF protein stained by p14ARF antibody (GTX129903) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation of p14ARF protein from HeLa whole cell extracts using 5 μg of p14ARF antibody (GTX129903).
Western blot analysis was performed using p14ARF antibody (GTX129903).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

p14ARF antibody detects p14ARF protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody (GTX129903) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Cancer Cell Int in 2020.PMID: 31920463