Human colon (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CEACAM1 antibody [N1N3] (GTX113392) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

C-CAM1 antibody [N1N3] detects C-CAM1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
C-CAM1 antibody [N1N3] (GTX113392) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min