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CERCAM antibody

Anti-CERCAM antibody used in Western Blot (WB). GTX123293
Anti-CERCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX123293
Anti-CERCAM antibody used in Western Blot (WB). GTX123293
Anti-CERCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX123293

Cat. No. GTX123293

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CERCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

GL261 , C8D30 , NIH-3T3 , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CERCAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cerebral endothelial cell adhesion molecule , CEECAM1 , GLT25D3

Cellular Localization

Endoplasmic reticulum lumen

Background

Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity.

Database

Research Area

DATA IMAGES

Anti-CERCAM antibody used in Western Blot (WB). GTX123293

GTX123293 WB Image

CERCAM antibody detects CERCAM protein by Western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
7.5 % SDS-PAGE
CERCAM antibody (GTX123293) dilution: 1:500

Anti-CERCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX123293

GTX123293 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using CERCAM(GTX123293) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CERCAM antibody used in Western Blot (WB). GTX123293

GTX123293 WB Image

CERCAM antibody detects CERCAM protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
CERCAM antibody (GTX123293) dilution: 1:500

Anti-CERCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX123293

GTX123293 IHC-P Image

CERCAM antibody detects CERCAM protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
CERCAM antibody (GTX123293) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lin CY et al. Sci Adv 2023; 9 (14) : eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169