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CFDP1 antibody

KO/KD
Anti-CFDP1 antibody used in Western Blot (WB). GTX128023
Anti-CFDP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128023
Anti-CFDP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128023
Anti-CFDP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128023
Anti-CFDP1 antibody used in Western Blot (WB). GTX128023

Cat. No. GTX128023

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CFDP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CFDP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885678

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

craniofacial development protein 1 , BCNT , BUCENTAUR , CENP-29 , CP27 , SWC5 , Yeti , p97

Background

May play a role during embryogenesis.

Database

Research Area

DATA IMAGES

Anti-CFDP1 antibody used in Western Blot (WB). GTX128023

GTX128023 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CFDP1 antibody (GTX128023) diluted at 1:3000.

Anti-CFDP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128023

GTX128023 ICC/IF Image

CFDP1 antibody detects CFDP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CFDP1 stained by CFDP1 antibody (GTX128023) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10μm.

Anti-CFDP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128023

GTX128023 IHC-P Image

CFDP1 antibody detects CFDP1 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
CFDP1 antibody (GTX128023) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CFDP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128023

GTX128023 IHC-Fr Image

CFDP1 antibody detects CFDP1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: CFDP1 stained by CFDP1 antibody (GTX128023) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-CFDP1 antibody used in Western Blot (WB). GTX128023

GTX128023 WB Image

CFDP1 antibody detects CFDP1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12 % SDS-PAGE
CFDP1 antibody (GTX128023) dilution: 1:1000

REFERENCE

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REVIEW

CFDP1 antibody Cat. No. GTX128023

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-CFDP1 antibody used in Western Blot (WB). GTX128023
Date : Anonymous submitted on 20-Jun-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : MC3T3-E1 cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169