CHD1L antibody immunoprecipitates CHD1L protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CHD1L protein by 2 μg of CHD1L antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated CHD1L protein was detected by CHD1L antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: HepG2
B: HepG2 nucleus
5% SDS PAGE
GTX119360 diluted at 1:3000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CHD1L antibody [N2C1], Internal (GTX119360) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.