CHD4 antibody detects CHD4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
CHD4 antibody (GTX124186) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX124186).
Western blot analysis was performed using CHD4 antibody (GTX124186) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using CHD4(GTX124186) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX124186) or CHD4 antibody (GTX101662).
Western blot analysis was performed using CHD4 antibody (GTX124186).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHD4(GTX124186) antibody at 1:500 dilution.

CHD4 antibody detects CHD4 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
CHD4 antibody (GTX124186) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX124186 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Int J Mol Sci in 2019. PMID: 31438571

The data was published in the journal Cell Discov in 2015.PMID: 27462424